| EHG Hotel Ltd | Hospitality | 2020 |
| Ekay Accounting Service Ltd | Professional & scientific | 2018 |
| Element Wellness Limited | Other services | 2024 |
| Elim Healthcare & Support Services Ltd | Public administration | 2026 |
| Elite Electrical & Renewables Ltd | Construction | 2025 |
| ELM Glasgow Project Limited | Construction | 2024 |
| EM Rentals Limited | Real estate | 2023 |
| Emuworks Limited | Manufacturing | 2023 |
| Enodis Consulting Ltd | Professional & scientific | 2021 |
| Enspire Architecture Studio Ltd | Professional & scientific | 2021 |
| Epkeng Ltd | Professional & scientific | 2026 |
| ERJ Autos Ltd | Wholesale & retail | 2023 |
| Erradale Properties Ltd. | Real estate | 1999 |
| Escape Stirling (Arcade) Ltd | Other services | 2023 |
| Escape Stirling Ltd | Other services | 2020 |
| ESP Property Investment Solutions Ltd | Real estate | 2021 |
| Estheva Limited | Other services | 2020 |
| European Turfgrass Laboratories Limited | Professional & scientific | 1996 |
| Evalina Ltd | Administrative & support | 2018 |
| Event Genies Ltd | Administrative & support | 2020 |
| Events by Plant OUT Ltd | Administrative & support | 2024 |
| Everyday Retail Limited | Wholesale & retail | 2025 |
| Exhibit Antiques & Collectibles Ltd. | Wholesale & retail | 2023 |
| EZE Events Ltd. | Other services | 2014 |
| F1X Auto Ltd | Wholesale & retail | 2025 |
| Fair Winds Trust | Transport & logistics | 2008 |
| Fairmount Rose Holdings Ltd | Professional & scientific | 2024 |
| Fanboy Limited | Wholesale & retail | 2020 |
| Fast & Fast Couriers Ltd | Transport & logistics | 2025 |
| Fife Drive School of Motoring Ltd | Other services | 2025 |
| Finlarig Ltd. | Real estate | 1999 |
| Fish Pharm Supplies Limited | Professional & scientific | 1985 |
| Fitness Solutions Scotland Limited | Manufacturing | 2002 |
| Flexetec Ltd | Manufacturing | 2020 |
| Flosverse Techsphere C.I.C. | Information & communication | 2023 |
| Flourish Home Support Services Ltd | Health & social care | 2011 |
| Ford Financial Planning Ltd | Financial services | 2024 |
| Forefront Productions (Alloa) Limited | Arts & recreation | 2014 |
| Forest JKM Ltd Limited | Agriculture & fishing | 2020 |
| Forth Access Services Ltd | Professional & scientific | 2025 |
| Forth Valley Recruitment Solutions Limited | Administrative & support | 2024 |
| Forum Property Developments Ltd | Information & communication | 2013 |
| FOZ Holdings Ltd | Financial services | 2019 |
| Fozsports Claims Limited | Financial services | 2019 |
| Fozsports Limited | Wholesale & retail | 2006 |
| Freddys Cabs Garage Services Ltd | Wholesale & retail | 2025 |
| Freedom Fuelled Ltd | Arts & recreation | 2024 |
| Fresh on the Go Scotland Limited | Extraterritorial | 2014 |
| Frixtex Limited | Other services | 1985 |
| Fusion IT Systems Limited | Information & communication | 2018 |
| Fusion Spice Alloa Limited | Hospitality | 2018 |
| Fusion-Synth Technologies Ltd | Information & communication | 2024 |
| FV Fitness Ltd | Arts & recreation | 2025 |
| FW Vehicle Solutions Limited | Administrative & support | 2018 |
| Fyfe Mcwilliam Ltd | Administrative & support | 2024 |
| G.J. Nimmo Electrical Contractors Limited | Construction | 2003 |
| G.M Welding Services Artistic Ironworks Ltd. | Manufacturing | 2016 |
| Game of Throwing (Stirling) Ltd | Arts & recreation | 2022 |
| Gang Forward MMA Ltd | Wholesale & retail | 2023 |
| Garden Energy Ltd | Energy | 2021 |
| Gartmorn Retail Limited | Wholesale & retail | 2015 |
| Gdall Engineering Ltd | Professional & scientific | 2019 |
| Geddes Consultancy Group Ltd | Professional & scientific | 2022 |
| Geek Sanctuary Ltd | Information & communication | 2025 |
| Geldis Ltd | Hospitality | 2010 |
| Geosonic Drilling Limited | Construction | 2012 |
| Geosonic Plant Limited | Administrative & support | 2012 |
| Geosonic Property Limited | Real estate | 2015 |
| Geostokos (Ecosse) Limited | Mining & quarrying | 1999 |
| Glass Repair Scotland Ltd | Construction | 2017 |
| Glenallan Gifts Ltd | Wholesale & retail | 2025 |
| Glengordon Software Limited | Information & communication | 1989 |
| Gloam Creative Limited | Administrative & support | 2017 |
| GM Fitness Bootcamp Limited | Arts & recreation | 2018 |
| Gold Star Comps Ltd | Arts & recreation | 2025 |
| Golden Tree Estates Ltd | Real estate | 2022 |
| Good Buys Ltd | Wholesale & retail | 2023 |
| Gorangrey Ltd | Agriculture & fishing | 2023 |
| Grangemouth Retail Properties Limited | Real estate | 2018 |
| Gravel Shed Limited | Extraterritorial | 2000 |
| Great Britain Footgolf CIC | Arts & recreation | 2024 |
| Great Hong Kong Alloa Ltd | Hospitality | 2021 |
| Green Breeze Energy (Holdings) Ltd | Energy | 2016 |
| Green Breeze Energy Ltd | Energy | 2012 |
| Greenfields Design Limited | Professional & scientific | 1993 |
| Greenpower (Argyll Holdings) Ltd | Energy | 2024 |
| Greenpower (Argyll) Ltd | Energy | 2024 |
| Greenpower (Barachander) Ltd | Energy | 2024 |
| Greenpower (Carie) Limited | Energy | 2014 |
| Greenpower (Carraig Gheal) Limited | Energy | 2003 |
| Greenpower (Fordoun) Limited | Energy | 2014 |
| Greenpower (International) Ltd. | Administrative & support | 2000 |
| Greenpower (Joint Ventures) Ltd | Administrative & support | 2016 |
| Greenpower (Wind) Ltd | Energy | 2011 |
| Greenpower Developments Ltd. | Administrative & support | 2001 |
| Greenpower EV Ltd | Administrative & support | 2021 |
| Greenpower Hydrogen Ltd | Administrative & support | 2021 |
| Greenways Ecology Ltd | Professional & scientific | 2025 |
| Gromax Limited | Wholesale & retail | 2024 |
| Group Taylor-Duncan Ltd | Financial services | 2026 |