| Eurosteel Structures Ltd | Construction | 2023 |
| Eventsplus Hospitality Ltd | Administrative & support | 2024 |
| Ever Green Grocers Ltd | Wholesale & retail | 2024 |
| Evi's Kitchen Limited | Hospitality | 2024 |
| Evita Healthcare International Ltd | Health & social care | 2024 |
| Evotec Racing Development Ltd | Other services | 2024 |
| Excelsior Cryogenic Pump Support Limited | Mining & quarrying | 2023 |
| Excelsior Driving School Ltd | Education | 2026 |
| Excelsior Events UK Ltd | Administrative & support | 2025 |
| Excelsior Stadium Limited | Arts & recreation | 2008 |
| Excelsior Stadium LLP | | 2007 |
| Exsells Trading (Private) Limited | Wholesale & retail | 2020 |
| Eyecon Precision Limited | Professional & scientific | 2016 |
| EZF Properties Ltd | Real estate | 2024 |
| EZY Fit Tyres Ltd | Wholesale & retail | 2025 |
| FA Consultancy Limited | Financial services | 2018 |
| FAB Recruitment Ltd | Administrative & support | 2026 |
| Fairdeal Licenced Grocers Ltd. | Wholesale & retail | 2014 |
| Fairleys Ices Ltd | Hospitality | 2026 |
| FAR Futures Ltd | Real estate | 2023 |
| Farayi Care Limited | Public administration | 2025 |
| Fashion & Beauty Ltd | Other services | 2019 |
| Fastfit Airdrie Limited | Administrative & support | 1991 |
| Fastsigns Revive Ltd | Manufacturing | 2025 |
| Feature Walls Scotland Limited | Construction | 2022 |
| Feeley Logistics Ltd | Transport & logistics | 2026 |
| Feline Elegance at Songbird Cattery Limited | Other services | 2024 |
| Felix Travers & Sons Ltd. | Wholesale & retail | 2003 |
| Ffdesign (Scotland) Ltd | Professional & scientific | 2025 |
| FGR Golf Limited | Wholesale & retail | 2018 |
| Filip Trans Limited | Transport & logistics | 2015 |
| Finnigan Joiners Ltd | Construction | 2011 |
| Fiordara Limited | Wholesale & retail | 2024 |
| Fire Line Systems Ltd | Manufacturing | 2024 |
| Firpark Properties Limited | Real estate | 2015 |
| First Generation Family CIC | Education | 2025 |
| First Principles Early Years Consultancy Limited | Education | 2021 |
| First Principles Nursery Group Ltd | Education | 2025 |
| Fitness Takeaway Limited | Administrative & support | 2011 |
| Five Horses Ltd | Wholesale & retail | 2026 |
| Flairplan Limited | Wholesale & retail | 2018 |
| Flamingo Nails & LUX Spa Ltd | Other services | 2024 |
| Flanigan Joinery Limited | Construction | 2020 |
| Flanigan Projects Holdings Limited | Real estate | 2024 |
| Flanigan Properties Ltd | Real estate | 2026 |
| Flanigan Technical Services Ltd | Agriculture & fishing | 2023 |
| Flanigan Tree & Land Services Limited | Administrative & support | 2020 |
| Flat OUT Nutrition Ltd | Health & social care | 2024 |
| Flatout Automotive Group Ltd | Financial services | 2025 |
| Flatout Performance Ltd | Wholesale & retail | 2018 |
| Flatout Property Group Ltd | Financial services | 2025 |
| Flatpack Media Limited | Information & communication | 2021 |
| Flawless Faces Beauty Aesthetics Ltd | Other services | 2024 |
| Fleet BID Ltd | Administrative & support | 2017 |
| Fleur DE Galerie Ltd | Professional & scientific | 2025 |
| Flow State Consulting Ltd | Professional & scientific | 2025 |
| Flynn Civil Engineering Ltd | Construction | 2020 |
| FNP Property Limited | Real estate | 2021 |
| Focus World UK Ltd | Wholesale & retail | 2016 |
| Follano's Limited | Wholesale & retail | 2012 |
| Food Choice Scot Ltd | Hospitality | 2026 |
| Forbes Sales Co Ltd | Wholesale & retail | 2024 |
| Ford Forge Properties Ltd | Other services | 2006 |
| Forgewell Holdings Limited | Professional & scientific | 2022 |
| Forgie Luxury Travel Ltd | Transport & logistics | 2021 |
| Fortem Flooring Ltd | Construction | 2016 |
| Forth Kitchens & Bathrooms Ltd | Other services | 2013 |
| Forth Valley Contracts Ltd. | Construction | 2010 |
| Forth Valley Developments Ltd. | Construction | 2010 |
| Foundry Warehousing Ltd | Financial services | 2026 |
| Fouro Creative Ltd | Information & communication | 2016 |
| FOX Construction (Holdings) Limited | Construction | 1997 |
| Foxy Floors Ltd | Construction | 2018 |
| Fpdc Limited | Construction | 2026 |
| FPM Consultancy Limited | Information & communication | 2014 |
| FRACTAL369 Ltd | Education | 2022 |
| Fraser M Bryce Investments Ltd | Wholesale & retail | 2004 |
| Fraser Plumbing and Heating Limited | Construction | 2019 |
| Frbc Rooofers and Builders Ltd | Extraterritorial | 2024 |
| Freeborn Motorcycle Club Limited | Other services | 2023 |
| Freedom Campervans (Scotland) Ltd | Hospitality | 2018 |
| Fresh Spuds Ltd | Hospitality | 2024 |
| Frontier Fencing Contractors Ltd | Construction | 2024 |
| Frro Consultancy Ltd | Professional & scientific | 2025 |
| Fruehauf (GBR) Ltd | Administrative & support | 2022 |
| Fruitfield Limited | Construction | 2024 |
| Fuko and Luna Dog Grooming Ltd | Other services | 2016 |
| Furbox Group Ltd | Wholesale & retail | 2018 |
| Furore Limited | Real estate | 2020 |
| Furreal Limited | Real estate | 1985 |
| Fury Garage Ltd | Wholesale & retail | 2023 |
| Fusion Electrical and Civils Ltd | Construction | 2007 |
| Future Group Scotland Ltd | Construction | 2024 |
| FX Logistics Ltd | Transport & logistics | 2023 |
| G & C Public Houses Limited | Hospitality | 2023 |
| G & G Engineering Supplies Ltd | Manufacturing | 2019 |
| G & N Carmichael | | 1999 |
| G FIX Joinery Ltd | Construction | 2024 |
| G Keenan Electrical Services Ltd | Construction | 2024 |
| G M Body Repairs Ltd. | Other services | 2019 |