| Rolink Maintenance Limited | Construction | 2011 |
| Romache Ltd | Construction | 2023 |
| Roman Photography Limited | Other services | 2007 |
| Ronald G. Johnston Limited | Wholesale & retail | 1959 |
| Rooco Limited | Real estate | 2026 |
| Rope Access Plains Ltd | Construction | 2025 |
| Rose Room Candles and Gifts Limited | Other services | 2017 |
| Roseland CSI Ltd | Professional & scientific | 2021 |
| Rossi & SON Limited | Real estate | 1937 |
| Rowantree Property Services Limited | Real estate | 2010 |
| RP Contracts Ltd | Construction | 2017 |
| RR GAS Services Ltd | Construction | 2017 |
| RS Comms Services Ltd. | Other services | 2017 |
| RS Contract Services Ltd | Information & communication | 2021 |
| RTM Corporation Ltd | Wholesale & retail | 2023 |
| Rubix Paving Ltd | Construction | 2024 |
| Rumpelstiltskin Ltd. | Other services | 2019 |
| Russell Homes Limited | Construction | 2004 |
| Rvuk Ltd | Information & communication | 2021 |
| RWA Electrical Ltd | Construction | 2025 |
| S & a Joiners Ltd | Construction | 2026 |
| S & L Barbour Investments Ltd | Financial services | 2018 |
| S C Groundworks (Scot) Ltd | Construction | 2026 |
| S M Management Consultants Limited | Professional & scientific | 2014 |
| S&C Contractors Ltd | Construction | 2025 |
| S&H Building Services Ltd | Construction | 2026 |
| S&S 509 Ltd | Wholesale & retail | 2024 |
| S-Dent Ltd | Health & social care | 2018 |
| S.A.M.S. (Scotland) Ltd. | Construction | 2011 |
| S2 BM Properties Ltd | Construction | 2023 |
| SA91 Ltd | Wholesale & retail | 2025 |
| Salen Holdings Limited | Real estate | 2021 |
| Saltire Interiors UK Ltd | Construction | 2025 |
| Saltire Transport Limited | Transport & logistics | 2025 |
| Sam's Travel Limited | Transport & logistics | 2015 |
| Samak Healthcare Ltd | Administrative & support | 2022 |
| Sanam & Anmol Ltd | Wholesale & retail | 2013 |
| Sano Wellbeing Ltd | Administrative & support | 2020 |
| Sapkotakharel Limited | Real estate | 2026 |
| Sartak Barber Limited | Other services | 2024 |
| Sarwar Enterprise Limited | Wholesale & retail | 2014 |
| SAS Towing Ltd | Transport & logistics | 2021 |
| Sataos Limited | Hospitality | 2026 |
| Sava Investments Limited | Real estate | 2024 |
| Save RX Ltd | Health & social care | 2026 |
| Saveco Properties Ltd. | Real estate | 2001 |
| SB Contracts (Scotland) Limited | Construction | 2023 |
| SB Power Engineering Limited | Manufacturing | 2024 |
| SBS Home Store Ltd | Wholesale & retail | 2019 |
| SC Site Services (Scotland) Ltd | Construction | 2019 |
| Scaffaccess (Scot) Ltd | Construction | 2021 |
| Scale My Business Ltd | Professional & scientific | 2025 |
| SCG Supplies Limited | Wholesale & retail | 2007 |
| Scotfix Limited | Wholesale & retail | 1989 |
| Scotia Extentions Limited | Construction | 2011 |
| Scotia PUB Limited | Hospitality | 2025 |
| Scotia Vehicle Inspection Limited | Wholesale & retail | 2026 |
| Scotia Vehicle Solutions Ltd | Other services | 2012 |
| Scotsafe Safety Solutions Ltd | Professional & scientific | 2014 |
| Scott & Co. Motor Engineers Ltd. | Wholesale & retail | 2019 |
| Scott Clelland Golf Technology Ltd | Wholesale & retail | 2018 |
| Scott Paints SC Limited | Wholesale & retail | 2004 |
| Scottish Logistics Training Academy Ltd | Education | 2023 |
| Scotty Brand Limited | Wholesale & retail | 2010 |
| Scran and Roadie Limited | Hospitality | 2022 |
| SCT Engineering Solutions (Airdrie) Ltd | Construction | 2013 |
| Seat Installations Limited | Construction | 2022 |
| Seerat Beauty and Massage Limited | Other services | 2025 |
| Sellorax Ltd | Wholesale & retail | 2026 |
| Seminar Solutions Ltd | Education | 2012 |
| Serendib Retail Glasgow Limited | Wholesale & retail | 2026 |
| SES Oliveres Ltd | Professional & scientific | 2025 |
| Seven Seas Exports S.I Ltd | Professional & scientific | 2024 |
| Sewco Limited | Wholesale & retail | 2005 |
| SF Ingress Solutions Ltd | Construction | 2026 |
| SGJ Perfumes Limited | Wholesale & retail | 2016 |
| SGM Training Ltd | Education | 2024 |
| SGS Studio Ltd | Arts & recreation | 2025 |
| SH Joiners Ltd. | Construction | 2008 |
| Shanks Consulting Limited | Professional & scientific | 2005 |
| Shanks Soils Ltd | Administrative & support | 2010 |
| Sharp Developments Limited | Construction | 2024 |
| Sharp Electrical Services Limited | Construction | 2026 |
| Shattered Reflections Limited | Professional & scientific | 2025 |
| Shaw Decorating Services Limited | Construction | 2022 |
| Shawburn Mini BUS Hire Ltd. | Transport & logistics | 1992 |
| Shell Carwash Ltd | Administrative & support | 2025 |
| Shewashan Ltd | Administrative & support | 2024 |
| Shfcom Limited | Information & communication | 2026 |
| Shibuya Library Ltd | Hospitality | 2026 |
| Shift Scotland Ltd | Transport & logistics | 2026 |
| Shifters Garage Ltd. | Wholesale & retail | 2016 |
| Shopvanta Ltd | Wholesale & retail | 2025 |
| Shunters (Coatdyke) Ltd | Hospitality | 2023 |
| SI Consultancy (Scot) Ltd | Administrative & support | 2026 |
| Sibbald Mechanical Services Ltd | Wholesale & retail | 2015 |
| Silver Cafe (Scot) Ltd | Hospitality | 2025 |
| Silver Star SOM Limited | Other services | 2015 |
| Silverdale Enterprises Ltd | Transport & logistics | 2015 |
| Simon Mclean Golf Ltd. | Arts & recreation | 2010 |