| Dominion Care Limited | Health & social care | 2022 |
| Donox Limited | Professional & scientific | 2013 |
| Donwhap Limited | Real estate | 2021 |
| Doormart Ltd | Wholesale & retail | 2026 |
| Doov Properties Limited | Real estate | 2025 |
| Dorothy Brooke Training Limited | Arts & recreation | 2020 |
| Dotonbori Ltd | Wholesale & retail | 2025 |
| Dotters Publishing Ltd | Information & communication | 2022 |
| Downey Healthcare Limited | Health & social care | 2009 |
| DPC Motorsport Limited | Professional & scientific | 2019 |
| DR Adrian Banning Limited | Health & social care | 2026 |
| DR Antonia Limited | Health & social care | 2025 |
| DR Iwase Ltd | Health & social care | 2013 |
| Dragon Express GAS Hvac Electric Service Ltd | Construction | 2015 |
| Dragons Property Solutions Ltd | Real estate | 2019 |
| Drain Technology (UK) Limited | Construction | 2005 |
| Drakanga Ltd | Wholesale & retail | 2023 |
| Dramco Limited | Wholesale & retail | 2004 |
| Draw 1 Design Ltd | Manufacturing | 2024 |
| Drayton Mill Management Company Limited | Households | 2002 |
| DRC Construction (Wantage) Limited | Construction | 2006 |
| Dream and Discover Ltd | Education | 2017 |
| Dream Spire Studios Ltd | Information & communication | 2021 |
| Dreams ARE Made Ltd | Arts & recreation | 2015 |
| Driftway Solutions Ltd | Professional & scientific | 2012 |
| Drivas Group Ltd | Transport & logistics | 2012 |
| Drivas Recruitment Ltd | Transport & logistics | 2011 |
| Driving Beyond Ltd | Education | 2024 |
| DRS Project Management Ltd | Professional & scientific | 2023 |
| Dryva Ltd | Professional & scientific | 2025 |
| DS Drinks Group Limited | Wholesale & retail | 2026 |
| DTF Contract Ltd | Information & communication | 2023 |
| DUA Innovations Ltd | Wholesale & retail | 2026 |
| DUB Catering Limited | Hospitality | 2011 |
| Ducatus Limited | Professional & scientific | 2014 |
| Duct Depot Ltd | Energy | 2025 |
| Duffield (Abingdon) Management Company Limited | Real estate | 1982 |
| Duma London Limited | Real estate | 2020 |
| Duratex UK Rubber & Plastics Ltd | Construction | 2009 |
| Dursun Yazilim Ltd | Wholesale & retail | 2025 |
| DW Trading Ltd | Information & communication | 2014 |
| Dymchurch Press Limited | Information & communication | 2023 |
| Dynamic Array Limited | Information & communication | 2007 |
| E&E 18 Limited | Hospitality | 2023 |
| E&M Cake and Coffee Ltd | Hospitality | 2023 |
| E.C. Ellis Property Ltd | Real estate | 2020 |
| E.R.Miller(Wantage)Limited | Real estate | 1957 |
| Eafrika-Public Relations & Development UK Ltd | Information & communication | 2012 |
| Eagle Contracting Ltd | Financial services | 2022 |
| Eagle Executive Chauffeurs Limited | Transport & logistics | 2010 |
| Ealing Property Holdings Limited | Real estate | 2017 |
| Ealing Property Management Limited | Real estate | 2019 |
| Earnest Audio Ltd | Arts & recreation | 2023 |
| Earth ALE Ltd | Manufacturing | 2014 |
| Earth Innovation Limited | Construction | 2019 |
| Earth Save Installations Ltd | Manufacturing | 2012 |
| Earth Silks Ltd | Wholesale & retail | 2024 |
| Earth Trust | Agriculture & fishing | 2002 |
| Earthvert Consulting Limited | Professional & scientific | 2022 |
| Easia CIC | Education | 2025 |
| East Wind Racing Limited | Arts & recreation | 1998 |
| Easybub Limited | Administrative & support | 2025 |
| EAT Your Own Limited | Administrative & support | 2014 |
| Ebrats Limited | Hospitality | 2024 |
| ECL Group Limited | Mining & quarrying | 1995 |
| ECM Business Services Ltd | Information & communication | 2015 |
| Eco Brick&Blockwork Ltd | Construction | 2024 |
| Eco Concepts and Solutions Ltd | Construction | 2024 |
| Eco Matters Renewable Assets Limited | Energy | 2016 |
| Eco-Synergy Oxon Ltd | Construction | 2024 |
| Ecocent Ltd Ltd | Extraterritorial | 2021 |
| Ecoconsult Limited | Professional & scientific | 2003 |
| Ecohives AI Ltd | Wholesale & retail | 2025 |
| Economic & Human Value Engineering Ltd | Professional & scientific | 2011 |
| Eddis Garage Ltd | Wholesale & retail | 2023 |
| Eden Mews Management Company Limited | Households | 1988 |
| Eden Research PLC | Professional & scientific | 1995 |
| Eden Rose Consultancy Services Ltd | Professional & scientific | 2019 |
| Edgbarne Trust Limited | Financial services | 1943 |
| Edinburgh International College Ltd | Education | 2009 |
| Edmorr Construction Company Ltd | Construction | 2022 |
| EDR Store Ltd | Wholesale & retail | 2025 |
| Education by Thomas Limited | Education | 2018 |
| Edwards Vehicle Services Ltd | Manufacturing | 2014 |
| Effet Decor & Events Ltd | Other services | 2022 |
| Efficient Electricians Ltd | Construction | 2018 |
| Eighty Foundation Ltd | Arts & recreation | 2022 |
| EJ Management Ltd | Professional & scientific | 2019 |
| EJL Construction (Oxford) Ltd | Construction | 2024 |
| Eklite Nail Surgery Ltd | Health & social care | 2024 |
| ELB Business Support Limited | Professional & scientific | 2021 |
| Elbereth Limited | Information & communication | 2016 |
| Elect Catering Supplies Limited | Administrative & support | 2021 |
| Electric Fusion Limited | Information & communication | 2024 |
| Electricncars Ltd | Manufacturing | 2020 |
| Electrogenos Ltd | Professional & scientific | 2022 |
| Elektra Software Ltd | Information & communication | 2019 |
| Element Windows Limited | Construction | 1986 |
| Elexion Consulting Ltd | Professional & scientific | 2024 |
| Elite Activity Group Ltd | Professional & scientific | 2025 |