| Rolf Park Farm Limited | Hants | 1976 |
| Rollakat Consulting Limited | York | 2017 |
| Rollaturf Limited | Middleton Tyas, Near Richmond | 2013 |
| Rolleston Dairy Limited | Bideford | 2026 |
| Rollestone Limited | Wiltshire | 2007 |
| Rollfields Limited | Grimsby | 2005 |
| Rolls-Strandmann Ltd | Cambridge | 2017 |
| ROM Fisheries and Camping Ltd | Glannau Dyfrdwy | 2025 |
| Romaheath Limited | Blackpool | 1993 |
| Roman River Beekeeping Company Ltd | Colchester | 2024 |
| Roman River Trees Ltd | Colchester | 2021 |
| Romance FC Ltd | London | 2019 |
| Romani Arnold Equine Ltd | Leeds | 2026 |
| Rome Farms Limited | Peterborough | 2012 |
| Romlily Pets Ltd | Seaham | 2024 |
| Romney Marsh Farms,Limited | Burmarsh Romney Marsh | 1947 |
| Romney Sheep Breeders Society (the) | Romney Marsh | 1895 |
| Romsey Tree Surgeons Limited | Romsey | 2009 |
| Romsey Trout Farm Limited | Hampshire | 1980 |
| RON Fields Nutrition Limited | Hereford | 2024 |
| RON Meadows Limited | Hale | 2007 |
| RON Morton Farming Limited | Ashbourne | 1997 |
| Ronald England & SON Limited | Shrewsbury | 1983 |
| Ronald Keith Agri Services Limited | Tayside | 2004 |
| Ronald Tough | Aberdeenshire | 1982 |
| Ronan Orla Limited | Herne Bay | 2015 |
| Ronchile Ltd | Kenilworth | 2026 |
| Rongbient Biosciences Limited | Norwich | 2025 |
| Ronin Mind Ltd | Ruislip, | 2021 |
| Ronnie Anderson Ltd | Lauder | 2020 |
| Roobeedoodle Ltd | Ilkeston | 2021 |
| Roof Tight Home Improvements NE Ltd | Darlington | 2019 |
| Rookery and Orchard Farms Limited | Petersfield | 1990 |
| Rookery Farm (Walcott) Limited | Norwich | 1998 |
| Rookery Farm Limited | Diss | 1996 |
| Rookery Farm Operations Ltd | Chinnor | 1996 |
| Rookery Farmers Limited | Wellingborough | 2015 |
| Rookery Open Farm Limited | Northampton | 2022 |
| Rookfield Limited | Newark | 2023 |
| Rookie Equestrian Limited | Woodstock | 2018 |
| Rookwood Farm Limited | Colchester | 2025 |
| Rookwoods Farm Limited | Bromsgrove | 2024 |
| Rooms Farm Limited | Market Drayton | 2015 |
| Rooney Properties Limited | Wisbech | 2016 |
| Rooster Farms Ltd | Baston | 2012 |
| Root and Pursuit Ltd | Bracknell | 2023 |
| Root Leaf and Bloom Ltd | Manchester | 2025 |
| Root One Central Ltd | Blanefield | 2018 |
| Root One Central Operations Ltd | Glasgow | 2024 |
| Root One West Ltd. | Glasgow | 2011 |
| Root Pouch (UK) Online Ltd | Whitworth | 2020 |
| Root Zero Norfolk Ltd | Wisbech | 2026 |
| Root's NFM Limited | Shepton Mallet | 2022 |
| Rootcause Ltd | New Malden | 2012 |
| Rootech Substrates UK Ltd | Stockport | 2026 |
| Rooted Chippenham Community Interest Company | Chippenham | 2022 |
| Rooted Community Food CIC | Polegate | 2019 |
| Rooted Futures Ltd | Pulborough | 2021 |
| Rooted in Bolton CIC | Bolton | 2023 |
| Rooted in Hull Limited | Hull | 2014 |
| Rooted Woodland Ltd | Derby | 2026 |
| Rooting 4 Change | Todmorden | 2025 |
| Rooting for YOU Ltd | Cheltenham | 2019 |
| Rootkin Associates Limited | Brecon | 1986 |
| Rootridge Limited | Bourne | 1986 |
| Roots & Bloom CIC | Sheffield | 2019 |
| Roots & Dreams Ltd | St. Albans | 2023 |
| Roots & KIN Ltd | Leeds | 2025 |
| Roots & Sprouts Ltd | Witham | 2021 |
| Roots & Wings Devon Ltd | Exeter | 2022 |
| Roots Agri Limited | Norwich | 2024 |
| Roots and Returns Limited | Peterborough | 2025 |
| Roots and Shoots Tree Surgery Limited | Charlesworth | 2025 |
| Roots and Shoots Tree Surveys Limited | Glossop | 2023 |
| Roots for the Future CIC | Godalming | 2016 |
| Roots Garden Nursery Limited | Maidenhead | 2009 |
| Roots Manoeuvred Limited | London | 2021 |
| Roots Ready Grow Ltd | Dundee | 2026 |
| Roots to Fruits CIC | Aldershot | 2023 |
| Roots to Shoots Ltd | Alcester | 2020 |
| Roots to the Moon Community Garden CIC | Robertsbridge | 2024 |
| Roots UP Tree Surgery Ltd. | Dunfermline | 2018 |
| Roots Upwards Limited | Brighton | 2003 |
| Rootvive Ltd | Harrow | 2025 |
| Rootz Plantlife CIC | Mildenhall | 2025 |
| Rope Access Tree Services Ltd | Cullompton | 2021 |
| Ropes and Slopes Limited | Willenhall | 2017 |
| Ropsley Farms Limited | Cheltenham | 1995 |
| Roque Limited | Godalming | 2014 |
| Rory Hanley Farriery Ltd | Hadleigh | 2021 |
| Rory Kemp Limited | Malton | 1965 |
| ROS Collins Dressage Ltd | Maidenhead | 2026 |
| ROS Farming Ltd | Taunton | 2022 |
| Rosan (Carms) Ltd | Carmarthen | 2024 |
| Rosap Services Ltd | Gressingham | 2019 |
| Roscar Angling Developments EU Limited | Worcester Park | 2013 |
| Rosconnor Dairies Ltd | Newry | 2024 |
| Roscourt Estates Limited | Dartford | 2015 |
| Rose (Eco) Ltd | Derby | 2021 |
| Rose a Farms Limited | Bishop Auckland | 2022 |