| Little Dale Dogs Limited | Southampton | 2026 |
| Little Danethorpe Farm Limited | Rossendale | 2016 |
| Little Derringstone Farm Ltd | Ashford | 1996 |
| Little Dowran Farm Limited | Penzance | 2021 |
| Little Droveway Stud Limited | Shipley | 2006 |
| Little Eaton Farm Chickens Ltd | Herne Bay | 2025 |
| Little Ethels Ltd | Blackpool | 2025 |
| Little Float Farm Limited | Dungannon | 2006 |
| Little Furs Cat Hotel Ltd | London | 2025 |
| Little Garden Wood Limited | Chipping Norton | 2007 |
| Little Gems Rescue Ltd | Bruton | 2016 |
| Little Green Seaweed Ltd | Penzance | 2023 |
| Little Hall Farms Limited | Essex | 1972 |
| Little Hamar Farm Company | Driffield | 2003 |
| Little Harbour Farm Equestrian Ltd | Chorley | 2022 |
| Little Harbour Farm Ltd | Chorley | 2020 |
| Little Hasguard Farm Limited | Haverfordwest | 2003 |
| Little Haven Farm Limited | Hedon | 2022 |
| Little HAY Partners Limited | Uttoxeter | 2017 |
| Little Heath Farm Nursery Ltd | Abbots Langley | 2019 |
| Little Hollands Limited | Eye | 2025 |
| Little Hunsley Farm Limited | Cottingham | 2009 |
| Little Karoo Farm Ltd | Maidenhead | 2019 |
| Little Kestle CIC | Newquay | 2025 |
| Little Kirkfield Farm Contracting Services Ltd | Huddersfield | 2025 |
| Little Lake View Track Livery Limited | Leicester | 2025 |
| Little Leaf Salads Ltd | Newport | 2025 |
| Little Legs Lamb Ltd | Helston | 2024 |
| Little Lodge Farm Limited | Droitwich | 2002 |
| Little London (Horses) Limited | Ashford | 1967 |
| Little London Stud Limited | Hassocks | 2019 |
| Little Long Legs Ltd | Camberley | 2025 |
| Little Mill Equestrian Limited | Carmarthen | 2021 |
| Little Mocho Eventing Limited | Childswickham | 2021 |
| Little Mongeham Limited | Deal | 1991 |
| Little Moor Allotments Ltd | Newcastle Upon Tyne | 2023 |
| Little Moss Farm Limited | Lanark | 2018 |
| Little Munden Equestrian Ltd | Aylesbury | 2013 |
| Little Nags Ltd | Thirsk | 2021 |
| Little Nevin Cattery Ltd | Wirral | 2014 |
| Little Oak Vineyard Ltd | Chipping Campden | 1998 |
| Little Orchard Alpacas Ltd | Axminster | 2016 |
| Little Paddocks Farm Limited | Alderley Edge | 2018 |
| Little Park Farm Devon Ltd | Exeter | 2021 |
| Little Penarth Farm Limited | Chepstow | 2004 |
| Little Pencoed Farms Limited | Cardigan | 2006 |
| Little PIG Brother Limited | Amersham | 2018 |
| Little Pigs at Home Animal Sanctuary CIC | Haverfordwest | 2022 |
| Little Rhoden Farm Limited | Canterbury | 1979 |
| Little River Management Limited | Southampton | 2010 |
| Little Rollright Estate (UK) Limited | London | 2002 |
| Little Rollright Farm Limited | London | 2015 |
| Little Rose Farm Limited | Leigh-On-Sea | 2022 |
| Little Staughton Farms Limited | Sandy | 1985 |
| Little Tykes Dog Hub Ltd | Grimsby | 2018 |
| Little Tylers Livery Ltd | West Horndon | 2026 |
| Little West END Farm Limited | Southampton | 2025 |
| Little Weston Orchard Limited | Bristol | 2025 |
| Little Wheekers Guinea PIG Rescue Bristol CIC | Bristol | 2025 |
| Little White Lamb Co Ltd. | Dereham | 2017 |
| Little Widefield Agricultural Holdings Limited | Okehampton | 2015 |
| Little Widefield Agricultural Land Limited | Leighton Buzzard | 2015 |
| Little Wilderness Ltd | Helston | 2020 |
| Little Windsor Farming Limited | Beaminster | 2001 |
| Little Witt Trading Company Limited | Edenbridge | 2010 |
| Little Wood Ayrshire Ltd | London | 2020 |
| Little Wycke Liveries Ltd | Benfleet | 2011 |
| Littlechild & SON Limited | Wisbech | 1942 |
| Littleport Mushroom Farms (2011) Limited | Ely | 2012 |
| Littleton Farm Limited | Dorset | 1964 |
| Littleworth Nurseries Limited | Spalding | 1985 |
| Litton Farming Limited | Bakewell | 2017 |
| Litton Farms Ltd | Radstock | 2025 |
| Litton Shoot Ltd | Bristol | 2018 |
| Littywood Farm Limited | Stafford | 2007 |
| Litua Ltd | Montrose | 2022 |
| Litwin Limited | Ely | 2014 |
| Live Fire Limited | Camborne | 2023 |
| Live Reef Limited | Tiverton | 2014 |
| Live Well Adventures Central Ltd | Ormskirk | 2023 |
| Livefood UK Limited | Somerset | 2003 |
| Liverpool Cotton Exchange Limited | London | 2023 |
| Liverpool Honey Company Ltd | Chorley | 2024 |
| Liverpool Tree Care Services Ltd | Liverpool | 2008 |
| Liversidge Contractors Ltd | York | 2020 |
| Liverton Farming Limited | Colchester | 2019 |
| Livery & Lettings Limited | Langho | 2017 |
| Livery Farm Enterprises Limited | Ballymoney | 2013 |
| Livesey Brothers Limited | Ashby-De-La-Zouch | 2021 |
| Livesoil Ltd | Hull | 2026 |
| Livestock (UK) Limited | Chester | 2014 |
| Livestock Biotech Ltd | Salford | 2025 |
| Livestock Improvement Corporation (UK) Ltd | Salisbury | 1983 |
| Livestock International Limited | Dymock | 2007 |
| Livestock Link Ltd | Stratford Upon Avon | 2002 |
| Livestock Management Systems (UK) Limited | Worcester | 2006 |
| Livestock Marketing (Borders) Limited | Roxburghshire | 1995 |
| Livestock Supplies Limited | Tarporley | 2003 |
| Livetec Services Limited | Canterbury | 2024 |
| Livetec Systems Limited | Canterbury | 2010 |