| Emmerdale Agriculture Ltd | London | 2023 |
| Emmerson and Co (Fishing) Limited | Bridlington | 2002 |
| Emmerson Breeders Limited | Altrincham | 2020 |
| Emmett Farming Limited | Taunton | 2012 |
| Emmett of Drayton Limited | Wallingford | 1953 |
| Emmotland Leisure Limited | Driffield | 2011 |
| Empawrium Dog Care Limited | East Kilbride | 2018 |
| Emperatrice Ltd | Wisbech | 2017 |
| Emperor K9 Academy Ltd | Coventry | 2023 |
| Emploshield Limited | Hartlepool | 2024 |
| Empowered Eventing Limited | Cirencester | 2023 |
| Empshott Farm Limited | Liss | 2021 |
| EMR AG Ltd | Bury St Edmunds | 2025 |
| EMR Farming Company | Nottinghamshire | 2007 |
| Emrd Ltd | Fraserburgh | 2018 |
| Emris Racing Ltd | St Ives | 2025 |
| Emroch Limited | Port Talbot | 2022 |
| EMS Equine Sport Therapy Ltd | Gloucester | 2024 |
| Emstree CYF | Cardiff | 2025 |
| EMV Fisheries Ltd | Wallingford | 2017 |
| EMW Equine Ltd | Newmarket | 2025 |
| ENB Services Limited | St. Helens | 2017 |
| Enborne Equestrian Community Interest Company | Newbury | 2014 |
| Enborne Equestrian Services Limited | Newbury | 2020 |
| Enchanted Bullies Limited | Barry | 2019 |
| Encompass Equine Solutions Ltd | Bury St. Edmunds | 2021 |
| Encouragemint Ltd | Birmingham | 2022 |
| Encrop Ltd | Wirral | 2019 |
| Enda Hughes Ltd | Newry | 2020 |
| Endeavour 17 Limited | Peterhead | 2003 |
| Endeavour Shellfish Ltd | Launceston | 2022 |
| Endocryne Limited | Edinburgh | 2019 |
| Endsleigh Fishing Club Limited | Perranwell Station Truro | 1962 |
| Energia Sustainable Solutions Limited | Ashford | 2012 |
| Energy C Limited | Scarborough | 2003 |
| Enfield VEG Co Limited | Enfield | 2014 |
| Enga Limited | Durham | 1989 |
| Engage Crop Solutions Ltd | Chorley | 2012 |
| Engelmann Farms Limited | Saffron Walden | 1961 |
| Engineering Service Woll-Gass Limited | Glasgow | 2016 |
| Englefield Green Limited | Slough | 2025 |
| English Guernsey Cattle Society(The) | Launceston | 1927 |
| English Oak Direct Limited | Hever Edenbridge | 1996 |
| English Sugar Beet Seed Company Limited | Lincolnshire | 1950 |
| English Woodlands Forestry Limited | Petworth | 2015 |
| Engulftek Ltd | Osmington | 2020 |
| Enhance Home Services Ltd. | Northwich | 2008 |
| Enigma Lakes Ltd | Malton | 2024 |
| Enjado Ltd | London | 2023 |
| Enjoy Fruitz Ltd | London | 2019 |
| Enmus Ltd | Surrey | 2015 |
| Ennis View Farms Limited | Enniskillen | 2018 |
| Ennislare Farm Limited | Armagh | 2025 |
| Enriched Being Ltd | Perth | 2023 |
| Ensigna Limited | Orpington | 2009 |
| Ensis Fishing Limited | Fort William | 2006 |
| Ensou Ltd | Oxford | 2018 |
| Entangled Earth Ltd | Taunton | 2021 |
| Entclem Limited | Cardiff | 2023 |
| Enterprise Investments WRI Limited | London | 2021 |
| Enterprise West Limited | Stockton-On-Tees | 1967 |
| Ento U.P Ltd | Malvern | 2021 |
| Entogrow Ltd | Kidderminster | 2023 |
| Entomics Biosystems Limited | Cambridge | 2015 |
| Entonexus Ltd | London | 2024 |
| Entosnack Ltd | Warrington | 2024 |
| Entwistle Farms Limited | Hoddleston | 2021 |
| Entwood Arboricultural Consultants Ltd | Lincoln | 2019 |
| Envi Group Ltd | Exeter | 2024 |
| Enviro Farms Tech Limited | London | 2020 |
| Enviro Tree Services Limited | Ormskirk | 2020 |
| Enviroarb-Solutions Limited | Colchester | 2015 |
| Envirofield Limited | Cheltenham | 2009 |
| Enviromac Contractors Limited | Ripon | 2025 |
| Environmental and Engineering Solutions Ltd | Beauly | 2018 |
| Environmental Farmers Group Limited | Hampshire | 2021 |
| Environmental Forestry UK Ltd | Haslemere | 2010 |
| Environmental Land Management and Education Ltd | Plymouth | 2025 |
| Environmental Regeneration Limited | Rotherham | 2020 |
| Environmental Rescue Limited | Chelmsford | 2020 |
| Envirotech NW Limited | Milnthorpe | 2004 |
| Envisage Groundcare Limited | Canterbury | 2021 |
| Envo Electronics Limited | Damerham | 2003 |
| Envol DE Retz UK Limited | Sheffield | 2024 |
| Enxus Limited | Loughborough | 2023 |
| Enziedi Ltd | Hawick | 2025 |
| EOS Contracting Limited | Manchester | 2013 |
| EP Logs Ltd | Telford | 2024 |
| EPC4BUILDINGS Ltd | Reading | 2007 |
| Epds Equine Limited | Hurst | 2014 |
| EPF Inspections Ltd | Omagh | 2016 |
| EPG Machine Tools Ltd | Enniskillen | 2020 |
| EPH Group Ltd | Tunbridge Wells | 2019 |
| Ephighland Limited | Edinburgh | 2025 |
| Ephraim Limited | Whitefield | 1925 |
| Epic Bullyz Limited | Birmingham | 2016 |
| Epic Tree Care Limited | Keith | 2015 |
| Epicure Community Market Garden CIC | Mildenhall | 2025 |
| Epigenetica Limited | Forfar | 2018 |
| EPL Summers Ltd. | Peterhead | 2008 |