| THE Regimental Store Limited | Wholesale & retail | 2013 |
| THE Rich Gordon Music Company Ltd | Information & communication | 2020 |
| THE Scotland Tours Company Limited | Administrative & support | 1997 |
| THE Secret Garden Outdoor Nursery | Education | 2006 |
| THE Snug at Logie Farm Ltd | Hospitality | 2021 |
| THE Spark Crew Ltd | Other services | 2012 |
| THE Sporting Pursuit Ltd | Professional & scientific | 2024 |
| THE ST. Andrews Consultancy Limited | Professional & scientific | 2005 |
| THE Tayview Bar Limited | Hospitality | 2023 |
| THE TIN Shed (Strength and Conditioning) Ltd | Arts & recreation | 2023 |
| THE Village Cafe - Ceres Community Cafe | Hospitality | 2019 |
| THE WEE Wild Company Ltd | Wholesale & retail | 2024 |
| Thistle Stays Limited | Hospitality | 2021 |
| Three Arches Wellbeing Ltd | Health & social care | 2026 |
| Tidy Gardens (Fife) Ltd | Administrative & support | 2008 |
| Tiger Properties (Scotland) Limited | Real estate | 2008 |
| TLC Environmental Limited | Professional & scientific | 2016 |
| TOD Hospitality & Entertainment Ltd | Hospitality | 2023 |
| Tonderghie Holdings Limited | Financial services | 2021 |
| Top Tyres (Fife) Limited | Wholesale & retail | 2019 |
| Torrential Potential Limited | Energy | 2010 |
| Treat Physiotherapy and Massage Ltd | Other services | 2024 |
| Triumph Over Injury Community Interest Company | Other services | 2018 |
| Tudu Risk Management Ltd | Professional & scientific | 2024 |
| Tuturu Limited | Manufacturing | 2018 |
| Tweeds Treats Limited | Manufacturing | 2024 |
| Two an a COO Ltd | Hospitality | 2023 |
| Urban India Ltd | Hospitality | 2024 |
| V2 Engineers Ltd. | Professional & scientific | 2003 |
| Veterans Tribe Scotland CIC | Wholesale & retail | 2021 |
| Veterinary Equine Dentistry Limited | Professional & scientific | 2018 |
| Viking Services Limited | Manufacturing | 2025 |
| VIM Media Limited | Information & communication | 2026 |
| Vintage Bespoke Designs Limited | Wholesale & retail | 2019 |
| Vionity Ltd | Information & communication | 2021 |
| Viridis (Scotland) Limited | Water & waste | 2025 |
| Virtusola Limited | Construction | 2022 |
| Vision Orthopaedics Ltd. | Health & social care | 2018 |
| VSS Scotland Ltd | Administrative & support | 2022 |
| Vyvfragrance Ltd | Manufacturing | 2021 |
| W by Design Ltd | Information & communication | 2025 |
| Walker Motors Limited | Wholesale & retail | 2011 |
| Wallace Holdings (Scotland) Ltd | Real estate | 2024 |
| Wanted Watches Ltd | Wholesale & retail | 2020 |
| Wardrop Associates Limited | Professional & scientific | 2013 |
| Wardrop Strategic Planning Limited | Professional & scientific | 2016 |
| Wayymark Outdoor Ltd | Other services | 2021 |
| Webdoc Limited | Information & communication | 2025 |
| Weddersbie Farm LLP | | 2010 |
| Welded Love Ltd | Manufacturing | 2023 |
| Wellburn Farm Ltd | Agriculture & fishing | 2023 |
| Wellfield Farms Limited | Agriculture & fishing | 1957 |
| West Hall Trancoso Limited | Financial services | 2011 |
| West Park Business Advisers Ltd | Professional & scientific | 2021 |
| What'Sup? Scotland Ltd | Education | 2024 |
| Whiteford Joinery Limited | Manufacturing | 2021 |
| Whole Cycle Ltd | Construction | 2019 |
| Widgewa Limited | Real estate | 2003 |
| Wild UX Ltd | Information & communication | 2013 |
| Wildredcroc LP | | 2016 |
| William AM Smith | Real estate | 2013 |
| Williamsgraywilliams Limited | Real estate | 2011 |
| Wills Oakden Training Ltd | Agriculture & fishing | 2023 |
| Wilson Steven Transport Limited | Transport & logistics | 2010 |
| Wilsons Paint & Bodyshop Ltd | Wholesale & retail | 2011 |
| Windward Education Limited | Hospitality | 2019 |
| WJM Consultancy Limited | Professional & scientific | 2016 |
| Woodhaven Consultants Limited | Professional & scientific | 2023 |
| Woodmill Game Limited | Agriculture & fishing | 1999 |
| Woodside Apartments Aberdour Ltd | Construction | 2026 |
| Wylie & Wood Bespoke Catering Limited | Hospitality | 2022 |
| Xcentuate Limited | Professional & scientific | 2011 |
| XEC Cars Ltd | Transport & logistics | 2021 |
| XEC Worldwide Ltd | Transport & logistics | 2019 |
| Young Diagnostics Ltd | Health & social care | 2020 |
| Zeco Refills & More Ltd | Wholesale & retail | 2022 |
| Zibu Designs Ltd | Wholesale & retail | 2025 |